rabbit polyclonal anti-ccr7 antibody  (Servicebio Inc)

Journal: Medicine

Article Title: Bioinformatics analysis reveals CCR7 as a potential biomarker for predicting CKD progression

doi: 10.1097/MD.0000000000033705

Figure Lengend Snippet: Top 10 upregulated and downregulated genes.

Article Snippet: After 3 TBS-T washes, membranes were incubated with the corresponding primary antibodies (rabbit polyclonal anti-CCR7 antibodies [Proteintech, 1:1000, Wuhan, Hubei, P.R.C], rabbit polyclonal anti-GAPDH, FN antibodies [Cell Signaling Technologies, 1:1000, Shanghai, China]) overnight at 4 °C.

Techniques:

Journal: Medicine

Article Title: Bioinformatics analysis reveals CCR7 as a potential biomarker for predicting CKD progression

doi: 10.1097/MD.0000000000033705

Figure Lengend Snippet: GO analysis of differentially expressed genes ( P < .05, count ≥ 4).

Article Snippet: After 3 TBS-T washes, membranes were incubated with the corresponding primary antibodies (rabbit polyclonal anti-CCR7 antibodies [Proteintech, 1:1000, Wuhan, Hubei, P.R.C], rabbit polyclonal anti-GAPDH, FN antibodies [Cell Signaling Technologies, 1:1000, Shanghai, China]) overnight at 4 °C.

Techniques: Chemotaxis Assay, Transduction, Cell Surface Receptor Assay, Binding Assay

Journal: Medicine

Article Title: Bioinformatics analysis reveals CCR7 as a potential biomarker for predicting CKD progression

doi: 10.1097/MD.0000000000033705

Figure Lengend Snippet: KEGG pathway enrichment analyses for differentially expressed genes ( P < .05, count ≥ 4).

Article Snippet: After 3 TBS-T washes, membranes were incubated with the corresponding primary antibodies (rabbit polyclonal anti-CCR7 antibodies [Proteintech, 1:1000, Wuhan, Hubei, P.R.C], rabbit polyclonal anti-GAPDH, FN antibodies [Cell Signaling Technologies, 1:1000, Shanghai, China]) overnight at 4 °C.

Techniques:

Journal: Medicine

Article Title: Bioinformatics analysis reveals CCR7 as a potential biomarker for predicting CKD progression

doi: 10.1097/MD.0000000000033705

Figure Lengend Snippet: Hub genes ranked in cytoHubba.

Article Snippet: After 3 TBS-T washes, membranes were incubated with the corresponding primary antibodies (rabbit polyclonal anti-CCR7 antibodies [Proteintech, 1:1000, Wuhan, Hubei, P.R.C], rabbit polyclonal anti-GAPDH, FN antibodies [Cell Signaling Technologies, 1:1000, Shanghai, China]) overnight at 4 °C.

Techniques:

Journal: Medicine

Article Title: Bioinformatics analysis reveals CCR7 as a potential biomarker for predicting CKD progression

doi: 10.1097/MD.0000000000033705

Figure Lengend Snippet: (A) Expression levels of CCR7, CCL21 , and CCL19 . (B) ROC curve analysis of CCR7, CCL21 , and CCL19 in 2 data sets. Significance is represented as * P < .05; ** P < .01; *** P < .00.

Article Snippet: After 3 TBS-T washes, membranes were incubated with the corresponding primary antibodies (rabbit polyclonal anti-CCR7 antibodies [Proteintech, 1:1000, Wuhan, Hubei, P.R.C], rabbit polyclonal anti-GAPDH, FN antibodies [Cell Signaling Technologies, 1:1000, Shanghai, China]) overnight at 4 °C.

Techniques: Expressing

Journal: Medicine

Article Title: Bioinformatics analysis reveals CCR7 as a potential biomarker for predicting CKD progression

doi: 10.1097/MD.0000000000033705

Figure Lengend Snippet: Correlation analysis of CCR7, CCL21, CCL19 with gender and age.

Article Snippet: After 3 TBS-T washes, membranes were incubated with the corresponding primary antibodies (rabbit polyclonal anti-CCR7 antibodies [Proteintech, 1:1000, Wuhan, Hubei, P.R.C], rabbit polyclonal anti-GAPDH, FN antibodies [Cell Signaling Technologies, 1:1000, Shanghai, China]) overnight at 4 °C.

Techniques:

Journal: Medicine

Article Title: Bioinformatics analysis reveals CCR7 as a potential biomarker for predicting CKD progression

doi: 10.1097/MD.0000000000033705

Figure Lengend Snippet: Expression levels of key genes in NRK-52E cells. Cells were treated with 10 ng/mL TGF-β1 for 0, 6, 12, 24, and 48 hours. (A) FN and CCR7 protein expression levels were analyzed by western blot. (B) FN and CCR7 mRNA levels were analyzed by real-time PCR. Results are presented as the mean ± standard error of 3 independent experiments; * P < .05; ** P < .01; *** P < .001 versus 0 hour.

Article Snippet: After 3 TBS-T washes, membranes were incubated with the corresponding primary antibodies (rabbit polyclonal anti-CCR7 antibodies [Proteintech, 1:1000, Wuhan, Hubei, P.R.C], rabbit polyclonal anti-GAPDH, FN antibodies [Cell Signaling Technologies, 1:1000, Shanghai, China]) overnight at 4 °C.

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction

Journal: Journal of Inflammation Research

Article Title: Chemokine CCL19 and Its Receptors CCR7 and CCRL1 in Chronic Rhinosinusitis

doi: 10.2147/JIR.S453567

Figure Lengend Snippet: Gene Expression Analysis in the Overall Study Cohort

Article Snippet: The tissues were then incubated with polyclonal rabbit anti-human CCR7 (1:200, 4°C, overnight) (Bioss Antibodies, bs-1305R; Woburn, MA, USA), followed by incubation with ImmPRESS TM HRP anti-rabbit IgG (20 min) and detection with ImmPACT DAB Peroxidase kits from Vector Laboratories (Burlingame, CA, USA).

Techniques: Expressing

Journal: Journal of Inflammation Research

Article Title: Chemokine CCL19 and Its Receptors CCR7 and CCRL1 in Chronic Rhinosinusitis

doi: 10.2147/JIR.S453567

Figure Lengend Snippet: Gene Expression Analysis with Respect to CRS Clinical Phenotype Compared to Controls

Article Snippet: The tissues were then incubated with polyclonal rabbit anti-human CCR7 (1:200, 4°C, overnight) (Bioss Antibodies, bs-1305R; Woburn, MA, USA), followed by incubation with ImmPRESS TM HRP anti-rabbit IgG (20 min) and detection with ImmPACT DAB Peroxidase kits from Vector Laboratories (Burlingame, CA, USA).

Techniques: Expressing

Journal: Journal of Inflammation Research

Article Title: Chemokine CCL19 and Its Receptors CCR7 and CCRL1 in Chronic Rhinosinusitis

doi: 10.2147/JIR.S453567

Figure Lengend Snippet: Spearman Correlations Between Genes in the Overall Study Cohort

Article Snippet: The tissues were then incubated with polyclonal rabbit anti-human CCR7 (1:200, 4°C, overnight) (Bioss Antibodies, bs-1305R; Woburn, MA, USA), followed by incubation with ImmPRESS TM HRP anti-rabbit IgG (20 min) and detection with ImmPACT DAB Peroxidase kits from Vector Laboratories (Burlingame, CA, USA).

Techniques:

Journal: Journal of Inflammation Research

Article Title: Chemokine CCL19 and Its Receptors CCR7 and CCRL1 in Chronic Rhinosinusitis

doi: 10.2147/JIR.S453567

Figure Lengend Snippet: Spearman Correlations Between Genes and CRS-Specific Disease Severity Metrics in the Overall Study Cohort

Article Snippet: The tissues were then incubated with polyclonal rabbit anti-human CCR7 (1:200, 4°C, overnight) (Bioss Antibodies, bs-1305R; Woburn, MA, USA), followed by incubation with ImmPRESS TM HRP anti-rabbit IgG (20 min) and detection with ImmPACT DAB Peroxidase kits from Vector Laboratories (Burlingame, CA, USA).

Techniques:

Journal: Journal of Inflammation Research

Article Title: Chemokine CCL19 and Its Receptors CCR7 and CCRL1 in Chronic Rhinosinusitis

doi: 10.2147/JIR.S453567

Figure Lengend Snippet: Sinonasal epithelial cell expression of CCL19 and its receptors CCR7 and CCRL1 is elevated in CRSwNP (n=7) compared to in CRSsNP (n=6) and controls (n=6). ( A ) Representative immunohistochemical images demonstrate the increased co-expression of CCL19 and typical receptor CCR7 (top), as well as of CCL19 and its atypical receptor CCRL1, in the sinonasal epithelium in patients with CRSwNP compared to in CRSsNP and controls. CCL19 appears gray, whereas its receptors appear dark brown. ( B ) CCL19 and CCR7 co-expression in the sinonasal epithelium, represented as the % total area via Image J analysis, is significantly elevated in CRSwNP compared to CRSsNP ( p =0.04) and controls ( p =0.06). ( C ) Although not significant, CCL19 and CCRL1 co-expression is elevated in CRSwNP compared to in CRSsNP and controls, represented as the % total area via Image J analysis. Data is represented by individual values with the mean ± SD with respect to cohort.

Article Snippet: The tissues were then incubated with polyclonal rabbit anti-human CCR7 (1:200, 4°C, overnight) (Bioss Antibodies, bs-1305R; Woburn, MA, USA), followed by incubation with ImmPRESS TM HRP anti-rabbit IgG (20 min) and detection with ImmPACT DAB Peroxidase kits from Vector Laboratories (Burlingame, CA, USA).

Techniques: Expressing, Immunohistochemical staining

Journal: Journal of Inflammation Research

Article Title: Chemokine CCL19 and Its Receptors CCR7 and CCRL1 in Chronic Rhinosinusitis

doi: 10.2147/JIR.S453567

Figure Lengend Snippet: Summary of Key Results

Article Snippet: The tissues were then incubated with polyclonal rabbit anti-human CCR7 (1:200, 4°C, overnight) (Bioss Antibodies, bs-1305R; Woburn, MA, USA), followed by incubation with ImmPRESS TM HRP anti-rabbit IgG (20 min) and detection with ImmPACT DAB Peroxidase kits from Vector Laboratories (Burlingame, CA, USA).

Techniques: Expressing